As shown in Figure 3, the percentage, absolute number (Figure 3(a)), and CD69 expression (Figure 3(c)) of Vγ4+ T cell in lungs quickly reached the peak at day 3 p.i. and kept a high level in absolute number at day 7 p.i., while the percentage, absolute number (Figure 3(b)), and CD69 expression (Figure 3(d)) of Vγ1+ T cells significantly increased at day 3 p.i. and reached the peak at day 7 p.i. Taking these results together, we concluded that Cm infection induced dramatic proliferation and activation of TCR Vγ4+ and Vγ1+ γδ T cells in the lungs at an early stage. This evidence concerns the gene CD69 and infection.