NEFM and neuromyelitis optica: At the clinical peak stage of NMO in the vehicle-treated rats, Western blotting (Supplementary Figures2C and2D) and immunostaining for NF-M (Figures 5, double stained with MBP), a marker of neurofilaments, revealed that the axonal density in the CNS was reduced (Figures 5(b) and 5(g); Supplementary Figures2C and2D) when compared with the control group (Figures 5(a) and 5(f); Supplementary2C and2D).