While in p53wt HCT116 cells, Z-DAN-11 caused a significant induction of apoptosis (>40% Annexin V+ve cells shown in Fig. 7a,b), in the p53−/− HCT116 cells it is significantly lower (around 27% Annexin V+ve cells), thereby confirming that p53 plays a definitive role in Z-DAN-11-mediated death of tumor cells. Here, TP53 is linked to neoplasm.