Because the proximal tubules are the tubule segment most vulnerable to cisplatin toxicity, to clarify the roles of DEPTOR in the pathology of AKI in vivo we used the Cre–LoxP system to generate conditional knockout (cKO) mice in which the DEPTOR gene was specifically deleted in the proximal tubule cells (Supplemental Fig. 1). Here, DEPTOR is linked to acute kidney injury.