TRIB2 and myeloid leukemia: To assess the impact of Trib2 deficiency on myeloid leukaemia cell survival, we measured the level of apoptosis following growth factor deprivation (GFD) and myeloid leukaemia-based chemotherapy (daunorubicin (DNR)) in the WT and Trib2-/- NH9 cells by flow cytometry using AnnexinV/DAPI staining (Fig. 2d–g).