The established SE-iFISH strategy, facilitated by the newly developed Metafer-iFISH® automated CRC image scanning and analysis system (Carl Zeiss, Oberkochen, Germany; MetaSystems, Altlussheim, Germany; and Cytelligen) [8], provides a unique comprehensive platform to effectively detect, phenotypically and karyotypically characterize CRCs with cytogenetic abnormalities of chromosome aneuploidy or gene rearrangements (such as ALK gene) in situ, in both patients and PDX tumor animal models. The gene discussed is ALK; the disease is colorectal carcinoma.