ALK and cancer: In addition to karyotypically examining aneuploidy of chromosomes in cancer cells, iFISH is also uniquely capable of qualitatively and quantitatively co-detecting multiple tumor biomarkers’ expression and cytogenetic gene rearrangement, such as anaplastic lymphoma kinase (ALK) gene [66] in adenocarcinomic non-small cell lung cancer (NSCLC) cells, as shown in Figure 3 (ALK-iFISH, Cytelligen, San Diego, CA, USA).