Since i•FISH® technology was reported for the first time on gastric CTC study [23], stepwise substantial improvement has been systematically built up to yield maximum efficiency and optimized flexibility for expeditious in situ co-detection of multiple tumor biomarkers or relevant proteins (such as PD-L1, CK, EpCAM, Vimentin, human epidermal growth factor receptor 2 (HER2), CD44, CD133, PSMA, GFAP, CD31, etc.), and aneuploidy of chromosome in CTCs at once [8,28,40,64]. Here, EPCAM is linked to neoplasm.