MYH6 and Duchenne muscular dystrophy: Immunolabeling of artificial muscles from hESCs and hiPSCs—healthy donor, Duchenne muscular dystrophy (DMD), limb-girdle muscular dystrophy type 2D (LGMD2D) (Figure S1C), and LMNA-related muscular dystrophies—showed homogeneous presence of myosin heavy chain (MyHC)+ multinucleated myotubes oriented along the force axis of the hydrogels (Figures 2A and 2B), as observed with primary human myoblasts within the same platform (Figure S1D).