TM - tumour marker. Group 1 – acridine-ester direct chemiluminescence. Group 2 - microparticle chemiluminescence. Group 3 - luminol/isoluminol chemiluminescence. Group 4 - flow fluorescence immunoassay. Group 5 – electrochemiluminescence. Group 6 - enzyme immunochemical luminescence. TEa - total allowable error. AFP - alpha-fetoprotein. CEA - carcinoembryonic antigen. PSA - total prostate specific antigen. CA125 - cancer antigen 125. CA15-3 - cancer antigen 15-3. CA19-9 – cancer antigen 19-9. The gene discussed is AFP; the disease is cancer.