MDA231 and MDA157 cells, either silenced for ZEB2 (si1-ZEB2, si2-ZEB2) or ectopically expressing miR-30a (5p/3p), were injected in the yolk sac of zebrafish embryos to monitor tumor cell spreading and in the cardinal vein (duct of Cuvier) to monitor tail fin invasion [31]. This evidence concerns the gene ZEB2 and neoplasm.