Depletion of neurofibromin in HeLa human epithelial carcinoma cells by transfection with a specific small interfering RNA (siRNA) induced a change in cell morphology from cuboidal to spindle-shaped, with reverse transcription (RT) and real-time polymerase chain reaction (PCR) analysis also revealing that depletion of neurofibromin increased the expression of mesenchymal marker genes including those encoding fibronectin (FN1) and N-cadherin (CDH2) as well as that of those for the EMT-TFs Slug (SNAI2) and Twist (TWIST1) (Supplementary Fig. S1). Here, TWIST1 is linked to carcinoma.