The tumour specimen of patient 1 was also positive for c.6772C>T, p.Arg2258X of NF1. Culture of the patient-derived neurofibroma cells and DFAT cells in the presence of various concentrations of tranilast revealed that the drug suppressed the growth of the cells in a concentration-dependent manner (Fig. 7a,b). This evidence concerns the gene NF1 and neurofibroma.