The immune responses to lymphocytic choriomeningitis virus (LCMV) and mouse CMV (MCMV) infections in C57BL/6 mice have been extensively characterized, and we used these infections as a model to illustrate the quality of our temperature-exchanged multimers in the detection of antigen-specific CD8+ T cells (Matloubian et al., 1994; van der Most et al., 1998; Rodriguez et al., 2001; Wherry et al., 2003). This evidence concerns the gene CD8A and infection.