As indicated in Fig. 1A and B, treatment with palmitate and LPS leads to the upregulation of Ifnb1 and Tnfa in murine hepatocytes (AML-12 cells) compared to the untreated control groups and C10 prevents LPS- and palmitate-induced upregulation of Ifnb1 and Tnfa. Treatment with C10 also prevents palmitate-induced pro-inflammatory cytokine expression in HepG2 cells, a human hepatocellular carcinoma cell line (Fig. 1C). This evidence concerns the gene TNF and hepatocellular carcinoma.