We next investigated the consequences of PIM inhibition in CLL cells using newly developed pan‐PIM inhibitor, SEL24‐B489.20, 25, 26 Incubation of CLL cells with 1‐10 μmol/L SEL24‐B489 for 24 hours caused a significant, dose‐dependent decrease in phosphorylation of PIM substrates: threonine 24/threonine 32 (T24/T32) of FOXO1/3a, serine 65 (S65) of 4EBP1 and serine 112 (S112) of BAD (Figure 3A‐C). The gene discussed is FOXO1; the disease is B-cell chronic lymphocytic leukemia.