For example, the G217R point mutation, first detected in inhibitor-resistant HCT116 human colon cancer cells, results in a 2,500-fold shift in the 50% effective concentration (EC50) of CHS-828 relative to parental HCT116 cells, with no associated change in the level of NAMPT protein [20]. The gene discussed is NAMPT; the disease is malignant colon neoplasm.