In this study, we showed that TGF-β induced EMT, that miR-30e was downregulated by TGF-β, and that miR-30e was predicted by bioinformatics analyses (TargetScan, TarBase, miRNA.org and MiRBase) to directly target the Snail 3′UTR, which we confirmed in CCA cells using Luciferase reporter assay. The gene discussed is TGFB1; the disease is cholangiocarcinoma.