CP and amyotrophic lateral sclerosis: Some GFP-TDP-43mNLS,C173S/C175S cytoplasmic aggregates in the CP were also immunoreactive to anti-phospho-TDP-43 (S409/S410) (Supplementary Fig. S7e) and to anti-ubiquitin antibodies (Supplementary Fig. S7f), consistent with the hyperphosphorylated and ubiquitinated status of aggregated TDP-43 in the spinal cord of ALS patients5–9.