RAB35 and neoplasm: Consistent, with this notion a dominant-negative RAB35S22N mutant abrogated PDGF-induced CDRs formation and directional migration (Supplementary Figure 3D), whereas two recently identified activated, tumour-associated RAB35 mutants, RAB35A151T and F161L48, promoted CDRs formation and elevated AKT phosphorylation in the absence of growth factor stimulation (Supplementary Figure 3E and Movie 16).