Our image analysis enables the extraction of valuable information such as COX deficiency levels – as an average ROD for the entire tissue or as a spectrum within the tissue (number of pixels per ROD); and variability within one specific genetic line [for example, we found that mtDNA mutator mice as well as Surf1 knockout mice varied substantially between each animal, whereas Lrpprc knockout and tRNAALA mice tended to be consistent (Figure 4E)]. The gene discussed is LRPPRC; the disease is hyperinsulinemic hypoglycemia, familial, 4.