Experimentally, overexpression of ZEB1-AS1 in the osteosarcoma cell line HOS via transfection with pcDNA3.1-ZEB1-AS1 plasmid results in enhanced cellular proliferation and migration, whilst knockdown of ZEB1-AS1 by transfection with short-hairpin (shRNA)-ZEB1-AS1 has the opposite effect. Here, ZEB1 is linked to osteosarcoma.