Galanin encoded by GAL is a neuroendocrine peptide, which is widely expressed in the central and peripheral nervous systems and also the gastrointestinal tract.31 Additionally, galanin modulates transmitter release from myenteric neurons via inhibition of voltage‐dependent calcium channels mediated by G‐protein‐coupled receptors.32 A recent genomewide study16 suggested GAL as a candidate for HSCR due to the reduced level of GAL expression in HSCR group compared with control group, and our results further supported this opinion. This evidence concerns the gene GAL and Hirschsprung disease.