To directly assess the role of YAP/TAZ in acute myeloid leukemia (AML) development, we took advantage of an established orthotopic mouse model of AML, based on the retroviral transduction of hematopoietic progenitor cells (HPCs) with the MLL-AF9 oncogene, alone or in combination with activated mutant NRAS (N-RASG12D) (Supplementary Figure 6a). Here, MLLT3 is linked to acute myeloid leukemia.