In addition, the significance of AFAP1-AS1 was also explored in hepatocellular carcinoma [16], cholangiocarcinoma [19], gastric cancer [23], esophageal squamous cell carcinoma [25], gallbladder cancer [27], ovarian cancer [28], tongue squamous cell carcinoma [29], and nasopharyngeal carcinoma [37]. This evidence concerns the gene AFAP1 and esophageal squamous cell carcinoma.