In order to investigate the expression of AFAP1-AS1 in retinoblastoma, the expression level of AFAP1-AS1 was detected by qRT-PCR in retinoblastoma tissue specimens, noncancerous retina tissue specimens, human retinoblastoma cell lines (Weri-Rb1 and Y79), ARPE-19 (human retinal pigment epithelial cell line), and HRMECs. Here, AFAP1 is linked to retinoblastoma.