To provide more evidence to characterize the negative regulation between miR-150-3p and SP1, the endogenous miR-150-3p was down-regulated by transfecting miR-150-3p antagomir into the glioma cells A172 and SHG-44, which harbored relatively higher expression of miR-150-3p amongst all the glioma cells we used. The gene discussed is SP1; the disease is central nervous system cancer.