TGFB1 and nephrocalcinosis: Upon anti-TGFβ antibody treatment, the number of renal pro-inflammatory macrophages was significantly reduced compared to IgG1-treated or untreated mice with nephrocalcinosis (Figure 4C), which was consistent with less intrarenal mRNA expression of inducible nitric oxide synthase (iNOS), a mediator of inflammatory responses (Figure 4D).