As shown in Fig. 2a, elevated CpG methylation levels of RUNX3 and TGF-β were detected in metastatic cancer tissues by qMSP, while reduced global DNA methylation levels of RUNX3 and TGF-β were observed compared with primary renal cancer tissues by quantitative methylation real-time PCR (Fig. 2b). The gene discussed is RUNX3; the disease is metastatic malignant neoplasm.