As our previous data showed that the high CpG methylation levels of RUNX3 and TGF-β played an important role in the metastasis ability of renal cancer, we next investigated whether decreased level of methylation by methylated transferase inhibitor methyltransferase (5-aza-2′-deoxycytidine) has an effect in the murine metastatic renal cancer xenograft model. Here, RUNX3 is linked to renal carcinoma.