To do this, we expressed the biosensor in three different cell lines: Human Umbilical Vein Endothelial Cells (HUVECs), MCF7 cells, a human breast adenocarcinoma cell line that does not express endogenous MMP1456, or MDA-MB-231 cells, a triple-negative human breast adenocarcinoma cell line with heightened MMP14 expression57–61. This evidence concerns the gene MMP14 and breast adenocarcinoma.