Several methods to count Ki67 positive and negative tumor cells exist, including eyeballing (i.e. best estimate) [9], counting 2000 cells in regions of interest (i.e. hotspots [8]) with the most frequent Ki67 nuclear labeling [10], counting Ki67 positive cells in 10 high power fields [11], or counting using automated image analysis (AIA) [5, 12]. This evidence concerns the gene MKI67 and neoplasm.