To establish conditions for knocking down neurofibromin protein, we treated three glioblastoma cell lines (T98G, SF268, and SF295) and non-transformed immortalized normal human astrocytes (NHA-E6/E7-hTert) with a SMARTpool of four siRNA constructs targeting the NF1 transcript (siNF1) optimized for target specificity, or a nontargeting SMARTpool control (siControl). Here, NF1 is linked to glioblastoma.