Of the 624 patients with non-squamous NSCLC who underwent R-EBUS transbronchial biopsy, 144 (23.1%) required repeat biopsies for pathological diagnosis or EGFR testing because of inaccessible transbronchial lesions (n = 6), negative transbronchial biopsy results (n = 107), or inadequate material (n = 31). This evidence concerns the gene EGFR and non-small cell lung carcinoma.