Furthermore, in vitro experiments in primary mouse hepatocytes and human hepatoma cell lines HuH7 and HepG2 together with in vivo experiments demonstrated that IL-1β exposure was sufficient to suppress transcription of Nr1h4, Abcb11, Abcc2, and Abcg5/8, findings that characterize the PNAC liver. This evidence concerns the gene IL1B and hepatocellular carcinoma.