To confirm the effect of BMP9 on bladder cancer cells, the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, the colony forming test, and the EDU (5-ethynyl-2′-deoxyuridine) test were used to determine the cell proliferation changes. The gene discussed is GDF2; the disease is urinary bladder carcinoma.