Besides the well-known hotspot mutations at codons 12, 13, and 61 (i.e., NRAS G13D, KRAS, and G12D/G13D), novel mutational sites have also been identified in ALL patients by using NGS methods (e.g., KRAS: V14I, V14L, K117N and A146T/P). The gene discussed is KRAS; the disease is acute lymphoblastic leukemia.