Additionally, none of the regulatory genes related to thermogenesis [42] was significantly changed in BAT by HFD feeding, including Ebf2, Ehmt1, Pparg, and Tle3. Therefore, our results indicated that expression of the aforementioned BAT thermogenic genes was not significantly affected by HFD-induced obesity. Here, PPARG is linked to obesity due to melanocortin 4 receptor deficiency.