We showed that abnormal p16 gene expression in bladder urothelial carcinomas correlated with the tumor grade and confirmed the results of other studies for a significant association between loss of p16 gene expression and urothelial carcinomas.[24,25] As reported previously,[31] p16 gene has a negative regulatory effect on bladder carcinogenesis at the early stage. Here, CDKN2A is linked to bladder transitional cell carcinoma.