The role of homocellular GJIC in glioma migration was highlighted in a recent report using a 3D spheroid migration model that mimics the in vivo architecture of tumor cells to quantify migration changes [75] and found that down-regulation of Cx43 expression in the U118 human glioma cell line increased migration by reducing cell–ECM adhesion, and changed the migration pattern from collective to single cell [75]. Here, GJA1 is linked to central nervous system cancer.