To evaluate whether the transduction enhancement observed with F13, H17, and K17 is independent of the transgene expressed, HEK293 cells were infected with dual AAV2 that express either ABCA4 (6.8 kb) or MYO7A (6.7 kb), which are causative of STGD1, the most common form of inherited macular degeneration, or USH1B, one of the most severe forms of inherited combined RP and deafness, respectively. The gene discussed is MYO7A; the disease is deafness.