The fluorescence pattern of ANA on HEp-2 cells provides additional diagnostic information: a pattern of multiple “nuclear dots” or a “nuclear rim” pattern suggest the presence of anti-sp100 or anti-gp210, respectively, and thereby hint at the diagnosis of PBC or a variant syndrome of AIH with features of PBC (see below section on autoantibodies in PBC). This evidence concerns the gene SP100 and primary biliary cholangitis.