TRPV4 and brain edema: In this study, we aim to investigate the following three hypotheses: (1) TRPV4 inhibition via pharmacological antagonists or a gene silencing approach ameliorates functional deficits and brain edema after experimental ICH in rats; (2) TRPV4 inhibition prevents stress fiber formation, degradation of the adherens and tight junction proteins and subsequent disruption of the BBB after ICH; (3) ICH induces the formation of stress fibers triggered by TRPV4 activation mediated by the PKCα/RhoA/MLC2 pathway.