The real-time PCR analysis revealed that the mRNA levels of matrix metallopeptidase 9 (MMP-9), plasminogen activator urokinase (uPA) and sushi domain containing 2 (SUSD2) that related to migration and cancer progression were significantly reduced in the pSi-STAT3 group, compared with the pSi-Scramble group after 72 h of transfection (Fig. 6B). This evidence concerns the gene PLAU and cancer.