In this study, miR-544 overexpression depressed the cytotoxicity of activated NK cells and the secretion of IFN-γ as well as the percent of NKp46+NK cells, on the contrary, synchronous overexpression of miR-544 and RUNX3 reversed the trends, revealing that miR-544 alleviated the cytotoxicity of NK cells, weakened the lethal ability of NK cells against liver cancer cells and sequentially mediated immune escape. This evidence concerns the gene NCR1 and liver cancer.