In this study, miR-544 overexpression depressed the cytotoxicity of activated NK cells and the secretion of IFN-γ as well as the percent of NKp46+NK cells, on the contrary, synchronous overexpression of miR-544 and RUNX3 reversed the trends, revealing that miR-544 alleviated the cytotoxicity of NK cells, weakened the lethal ability of NK cells against liver cancer cells and sequentially mediated immune escape. Here, RUNX3 is linked to liver cancer.