Thus, the observation that DFT1 and DFT2 both harbor predicted two-hit loss-of-function mutations in genes encoding proposed regulators and effectors of Hippo signaling (WWC3, MPDZ, TP73), together with evidence for activity of Hippo effectors YAP1 and WWTR1/TAZ in DFT1 and DFT2 cells, raises the possibility that this pathway is involved in DFT cancers in Tasmanian devils. Here, YAP1 is linked to cancer.