In this study, we sorted ICC (CopGFP-Kit+ cells) from small intestinal muscles of Kit+/copGFP mice by FACS from, as previously described (Baker et al., 2016), and characterized the expression of Chrm2 and Chrm3 transcripts and Tacr1 and Tacr2 transcripts. The gene discussed is CHRM3; the disease is intrahepatic cholangiocarcinoma.