Our results showed that the effect of PSMD7 depletion in ESCC cell lines was similar to MG132 treatment, but the decreased phosphorylation residue of p70S6K was different, which are Thr421/Ser424 in PSMD7 knockdown and Thr389 in MG132 treatment. This evidence concerns the gene RPS6KB1 and esophageal squamous cell carcinoma.