High-resolution 1H MRS of tumor extracts identified significant changes in some branched chain amino acids, organic acids, choline metabolites, substrates, and nucleotides in the aqueous phase, and triglycerides and lipids in the lipid phase of sh-HIF-1α, sh-HIF-2α and sh-HIF-1/2α tumors. This evidence concerns the gene EPAS1 and neoplasm.