In this study, the alteration of the inflammatory cells and total protein in BALF and also, gene expression and levels of TNF-α and IL-6, as important pro-inflammatory cytokines involved in immunoregulation and inflammation in lung tissue and BALF, was investigated for an in-depth evaluation of the inflammatory changes of lung in cigarette smoke induced COPD rat model. Here, TNF is linked to chronic obstructive pulmonary disease.