This method can simultaneously detect multiple DNA glycosylases with a detection limit of 2.23 × 10–6 U μL–1 for hOGG1 and 8.69 × 10–7 U μL–1 for hAAG without the involvement of any target amplification, and it can be used for the simultaneous measurement of enzyme kinetic parameters and the detection of hOGG1 and hAAG activities from lung cancer cells. This evidence concerns the gene OGG1 and lung cancer.