PC3 tumor cells transfected with or without plasmids pcDNA3.1-SALL1, pcDNA3.1-SALL4, and pcDNA3.1, were cultured at a starting number of 2 × 104/well in 24 wells (A), or 1 × 104/well in 96-well plates (B). The gene discussed is SALL4; the disease is neoplasm.