To determine the biological significance that TLR3 had on chlamydial shedding in mice bred in the C57BL/6N genetic background, we infected groups of five wild-type and five C57BL/6N TLR3-/- (referred as TLR3-/-) intravaginally with 105 IFU C. muridarum. As shown in Fig 1A, TLR3-/- mice bred in the C57BL/6 genetic background mice shed Chlamydia at significantly higher levels throughout the first 24 days of infection than did wild-type mice. Here, TLR3 is linked to infection.