WT1 and acute myeloid leukemia: Furthermore, next generation sequencing-based analysis revealed the existence of differentially methylated and hydroxymethylated regions between the wildtype AML controls and WT1 mutants, with the majority of 5hmC peaks localizing to enhancers and distal regulatory regions whereas differential 5mC peaks between wildtype and mutants clustered around transcriptional start sites (Rampal et al., 2014).