Next, using a SOCS2 reporter plasmid, SOCS2 promoter activity was measured in HEK293T cells after transfection with wild-type p53 or various mutant p53 constructs (Choi-CK p53, Cho-CK p53, JCK p53, and SCK p53), which were derived from the different cholangiocarcinoma cells20. Here, TP53 is linked to cholangiocarcinoma.